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Lab Exercise: Spectral Libraries

In this Laboratory You Will:

• Build a spectral library.

• Perform a library search.

You will need Class.M and Class.D created in the acquisition laboratory. If you

don’t have either of these files, use Isocra.m and one of the demo sequence files

that was created with this method.

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Agilent 1100/1200 HPLC 1
ChemStation Operation 1
Course Number H4033A 3
Laboratory Manual 3
Table Of Contents 5
ChemStation 9
In this Laboratory You Will: 10
Configuration Editor 13
Scheduler 14
Windows XP) 16
Cleaning up Temporary Files 16
Repairing the Disk 16
Disk Defragmentation 17
Workstation) 18
(Windows 2000 or XP) 21
Windows Explorer 21
Wallpaper and Screen Savers 22
Clipboard 23
Preparation 27
Preparing the HPLC 28
Practice Entering Gradients 32
OK the dialog box 34
Snapshot 35
Graphics - Signal Options 39
Graphics - Annotation 41
Window Functions 43
Peak Purity 44
Displaying Spectra 47
Isoabsorbance Plot 48
3D Plot 50
Building a Library 53
Library Searching 55
The Agilent 1200 Flow Path 59
Diagnosis View 61
Diagnosis Instrument Panel 62
Instrument Tests 64
Routine Maintenance 67
Agilent LC Diagnostic Tool 73
Diagnosing Common Problems 74
Instrument Status Report 77
Lab Exercise: Integration 81
Integration Preparation 83
Auto Integration 84
Adding Timed Events 87
Using Manual Events 89
Data Acquisition-Standards 97
Integration 99
Setting up Signal Details 101
Building a Calibration Table 102
Calibration Standards 104
Building a Sequence 109
Sequence Summary Reports 112
Starting the Sequence 113
Pausing a Sequence 114
Post Sequence 115
Reprocessing 117
Reprocess with DA Methods 125
Batch Review 129
Adding a Report Header 133
Automated Peak Purity 137
Preparations 143
Building a Customized Report 144
Adding a General Section 145
Inserting Tables 146
Adding a Calibration Curve 147
Finishing Up 148
Lab Exercise: Commands 149
In this LaboratoryYou Will: 150
Commands 152
Registers 153
Objects 154
Lab Exercise: Macro Writing 161
Write Your Own Macro 165
Possible Answer 166
Agilent ChemStation 168
Getting Started with New 168
ChemStation Workflow 168
In This Guide… 170
4 Data Organization 171
Contents 172
6 Data Organization 173
ChemStation Data Structure 174
ChemStation prior to B.02.01 175
ChemStation B.02.01/B.03.01 176
12 Data Organization 179
1 ChemStation Data Structure 179
Data Acquisition 180
18 Data Organization 185
2 Data Acquisition 185
Data Analysis 186
22 Data Organization 189
3 Data Analysis 189
B.02.01 and higher 189
Data Analysis: Data Review 190
Keep “Current Method” 191
Load “Sequence Method” 192
Update master method feature 196
Update Sequence Method 197
Master method — 197
Data Organization 33 200
Data Analysis 3 200
34 Data Organization 201
Agilent Technologies 202
42 Data Organization 209
*G2170-90041* 211

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