Agilent Technologies E5500 Series Guía de usuario descargar pdf (Pagina 71)

Fragment Library Preparation Using the AB Library Builder

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System: 5500 Series SOLiD

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Systems User Guide

AppendixD Library Construction Oligonucleotide Sequences

Barcoded adaptor sequences

Barcode-T-088, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTGTTGATCATGCTGCTGTACGGCCAAGGCGT-3′

Barcode-T-089, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTAGGCTGTCTACTGCTGTACGGCCAAGGCGT-3′

Barcode-T-090, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTGTGACCTACTCTGCTGTACGGCCAAGGCGT-3′

Barcode-T-091, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTGCGTATTGGGCTGCTGTACGGCCAAGGCGT-3′

Barcode-T-092, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTAAGGGATTACCTGCTGTACGGCCAAGGCGT-3′

Barcode-T-093, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTGTTACGATGCCTGCTGTACGGCCAAGGCGT-3′

Barcode-T-094, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTATGGGTGTTTCTGCTGTACGGCCAAGGCGT-3′

Barcode-T-095, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTGAGTCCGGCACTGCTGTACGGCCAAGGCGT-3

′

Barcode-T-096, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTAATCGAAGAGCTGCTGTACGGCCAAGGCGT-3′

Barcoded adaptor sequence Length (nt)

1 2 ... 66 67 68 69 70 71 72 73 74 75 76 ... 86 87

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Agilent Technologies E5500 Series Guía de usuario Pagina 71