Agilent Technologies 1100 Series Manual de usuario descargar pdf (Pagina 41)

Setting up an Analysis

Before Using the System

Solvent Information

Observe recommendations on the use of solvents in chapter “Solvents” in the

pump’s reference manual.

Priming and Purging the System

When the solvents have been exchanged or the pumping system has been

turned off for a certain time (for example, overnight) oxygen will re-diffuse

into the solvent channel between the solvent reservoir, vacuum degasser

(when available in the system) and the pump. Solvents containing volatile

ingredients will slightly lose these. Therefore priming of the pumping system

is required before starting an application.

Table 5 Choice of Priming Solvents for Different Purposes

Activity Solvent Comments

After an installation

When switching between reverse phase

and normal phase (both times)

Isopropanol

Best solvent to flush air out of the system

After an installation Ethanol or Methanol Alternative to Isopropanol (second choice)

if no Isopropanol is available

To clean the system when using buffers

After a solvent change

Bidistilled water

Best solvent to re-dissolve buffer crystals

After the installation of normal phase

seals (P/N 0905-1420)

Hexane + 5% Isopropanol Good wetting properties

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Agilent Technologies 1100 Series Manual de usuario Pagina 41