Agilent Technologies DP235 Guía de usuario Pagina 123

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Contents 123 Index
NOTE
With DNA and protein assays, the height of marker peaks is assay dependent. Ladder
peaks are analyzed to calculate a marker peak threshold that is used to locate the
marker peaks in the sample wells. If the marker peaks found using this calculated
method fail to align with those of a sample, the 2100 expert software will use the
minimum peak height threshold setting instead (if this value is lower than the value for
the marker peak). For example, the calculated threshold might be too high to find the
sample's markers if they happen to be very small for some reason. Either no markers
will be found or the wrong peaks will be assumed to be markers and these may not
align with the ladder markers. Consequently, the software attempts to use the
minimum peak height threshold that, if it is set low enough, will catch the real
markers, allowing the sample to align.
NOTE
After alignment, peaks are shown with relative migration times that are different from
the real times with data unaligned.
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