Agilent Technologies DP235 Guía de usuario descargar pdf (Pagina 177)

100

101

Contents ▲ 177 ▼ Index

Chip Reagents

Several reagents have to be added to the chip to prepare it for measurement. The

following image shows which reagents have to be filled in which wells.

Make sure you follow these directions when preparing the sample:

• The priming solution has to be added first. It fills all channels (removes all air from the

micro channels).

• The focusing dye is used to adjust the optic. The optics are focused horizontally and

vertically before each chip is measured.

• If you do not use all six wells, always load the first sample in well 1, the second sample

in well 2 etc. Unused wells have to be filled with cell buffer solution, otherwise they may

run dry during the chip run. Because all channels are connected to the priming well, this

may led to bubbles and to a clogging of the pressure adapter filter.

• The cell buffer is used to focus the cells before they pass the detection point. You have

to fill both buffer wells with cell buffer.

Priming solution

Cell buffer

Sample 1 – 6

Cell buffer

Focusing dye

solution

1 2 ... 172 173 174 175 176 177 178 179 180 181 182 ... 601 602

Comentarios a estos manuales

Sin comentarios

Agilent Technologies DP235 Guía de usuario Pagina 177

Comentarios a estos manuales

Relacionado con productos y manuales para Software Agilent Technologies DP235