Agilent Technologies DP235 Guía de usuario descargar pdf (Pagina 198)

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Contents ▲ 198 ▼ Index

Analyzing and Evaluating the Results of a Flow Cytometric Assay

You can analyze and evaluate result data of flow cytometric assays using either the dot

plot or the histogram view. In both views, you can evaluate the detected cells by defining

areas of interest.

• Histograms show the distribution of events related to the red and blue fluorescence

intensity. Gating is used to generate subsets based on markers in one histogram. See

“Using Histograms for Evaluation” on page 199 for detailed information.

• Dot plots show events as dots in a coordinate system where the blue fluorescence

value is related to the red. Regions and gates are used to determine the number of cells

with a fluorescence intensity lying in a defined range. See “Using Dot Plots for

Evaluation” on page 220 for detailed information.

If you use predefined assays, the markers and regions are set at the approximate position

where the events are expected. Refer to “Evaluating Antibody Staining, Apoptosis, and

GFP Assays” on page 229 for information on how to evaluate the predefined assays.

TIP

You can analyze and evaluate results already while a chip run is still in progress.

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