Agilent Technologies DP235 Guía de usuario Pagina 157
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Contents ▲ 157 ▼ Index
Principles of Flow Cytometric Measurements
Besides electrophoretic assays (DNA, RNA, and proteins), the Agilent 2100 bioanalyzer
supports flow cytometric assays:
• First, cells are stained with two fluorescent dyes that correspond to biologically
relevant parameters, as described in the application notes available for each assay.
“Staining Cells” on page 158 explains the principle.
• Next, the stained cells are analyzed on the chip. They pass the detector in single file and
are analyzed individually for their red and blue fluorescence intensities. The results are
displayed as histograms or dot plots. Refer to “Cell Detection with the Agilent 2100
Bioanalyzer” on page 160 for a detailed explanation.
- Agilent 2100 Bioanalyzer 1
- 2100 Expert User’s Guide 1
- Contents ▲ 2 ▼ Index 2
- Contents 3
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- About this Manual 6
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- In this Manual 7
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- Related Documents 9
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- What’s New in 2100 Expert 11
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- How to Use this Manual 13
- ▲ Displays the previous page 13
- ▼ Displays the next page 13
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- Quick Start 17
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- Looking at 2100 Expert 31
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- Starting 2100 Expert 32
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- 2100 Expert Work Area 33
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- Running a Demo Assay 41
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- Closing 2100 Expert 45
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- Cytometric Assays 47
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- 2100 Expert Data Overview 246
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- Handling Assays 249
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- Handling Chip Data 253
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- Importing Data 256
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- Exporting Data 263
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- Printing Reports 275
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- Configuring Tables 282
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- Reading the Log Books 287
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- Configuring 2100 Expert 293
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- How to Specify Graph Settings 298
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- Performing Qualifications 312
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