Agilent Technologies DP235 Guía de usuario Pagina 237

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Contents 237 Index
When using the calcein marker in the blue histogram for gating, only the living cells are
considered for building the red histogram. High red fluorescence values indicate living,
apoptotic cells, low red fluorescence values indicate living, non-apoptotic cells. See the
following example.
Low fluorescence value
indicates living non-apoptotic
cells
High fluorescence value
indicates living apoptotic cells
Percentage of all cells with
high red fluorescence
selected by the red marker
Amount of the living cells in relation to all measured cells
Amount of living cells with high red fluorescence in relation to the amount of living cells
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