Agilent Technologies 1100 Series Manual de instrucciones descargar pdf (Pagina 43)

1100 Series Capillary LC System Manual 33

Optimizing Performance 2

Solvent Information

Always filter solvents through 0.4 µm filters, small particles can permanently

block the capillaries and valves. Avoid the use of the following steel-corrosive

solvents:

• Solutions of alkali halides and their respective acids (for example, lithium

iodide, potassium chloride, and so on).

• High concentrations of inorganic acids like sulfuric and nitric acid,

especially at higher temperatures (replace, if your chromatography method

allows, by phosphoric acid or phosphate buffer which are less corrosive

against stainless steel).

• Halogenated solvents or mixtures which form radicals and/or acids, for

example:

• 2CHCl

+ O

→ 2COCl

+ 2HCl

• This reaction, in which stainless steel probably acts as a catalyst, occurs

quickly with dried chloroform if the drying process removes the stabilizing

alcohol.

• Chromatographic grade ethers, which can contain peroxides (for example,

THF, dioxane, di-isopropylether) such ethers should be filtered through dry

aluminium oxide which adsorbs the peroxides.

• Solvents containing strong complexing agents (e.g. EDTA).

• Mixtures of carbon tetrachloride with 2-propanol or THF dissolve stainless

steel.

• Avoid the use of alkaline solutions (pH > 8.5) which can attack the fused

silica from the capillaries.

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Agilent Technologies 1100 Series Manual de instrucciones Pagina 43