Agilent Technologies 1100 Series Manual de servicio descargar pdf (Pagina 31)

1100 Series DAD and MWD Reference Manual 31

How to optmize the Detector 2

Optimization Overview

Table 4 Optimization Overview

Parameter Impact

1 Selection of flow cell

• Choose flow cell according to used column, see Figure 7.

• peak resolution versus sensitivity

2 Connection of flow cell

• For flow rates from 0.5 ml/min connect column using the

zero-dead-volume fittings of the detector.

• For small column i.d. (e.g 1 mm) the inlet capillary of the micro flow cell

can be connected directly to the column.

• chromatographic resolution

3 Setting the peak width (response time)

• Use peak width according Figure 7 as starting point.

• Set the peak-width close to the width of a narrow peak of interest in

your chromatogram.

• peak resolution versus sensitivity versus

disk space

4 Setting wavelength and bandwidth

• Sample wavelength:

• Never miss a peak by the use of a browser wavelength like 250 nm

with 100 nm bandwidth.

• Select specific wavelength with reduced bandwidth if you need

selectivity, e.g. 250,10 nm and 360,100 nm as reference wavelength.

• Set the sample wavelength to a peak or valley in the spectrum to get

best linearity for high concentrations.

• Reference wavelength:

• Select the reference wavelength with broad bandwidth (30...100 nm)

wavelength range where your analytes have little or no absorbance

(e.g. sample at 254 nm, reference at 320 nm).

• sensitivity versus selectivity

• sensitivity versus linearity

• baseline drift due to RI effects.

1 2 ... 26 27 28 29 30 31 32 33 34 35 36 ... 317 318

Sin comentarios

Agilent Technologies 1100 Series Manual de servicio Pagina 31