Agilent Technologies 1100 Series Manual de servicio descargar pdf (Pagina 36)

36 1100 Series DAD and MWD Reference Manual

2 How to optmize the Detector

Sample and Reference Wavelength and Bandwidth

The detector measures absorbance simultaneously at wavelengths from 190 to

950 nm. Two lamps provide good sensitivity over the whole wavelength range.

The deuterium discharge lamp provides the energy for the UV range (190 to

400 nm) and the tungsten lamp emits light from 400 to 950 nm for the visible

and short wave near infrared.

If you know little about the analytes in your sample, use both lamps and store

all spectra over the full wavelength range. This provides full information but

fills up your disk space rather quickly. Spectra can be used to check a peak’s

purity and identity. Spectral information is also useful to optimize wavelength

settings for your chromatographic signal.

The detector can compute and store at run time up to 5 signals with these

properties:

• sample wavelength, the center of a wavelength band with the width of

sample bandwidth (BW), and optionally

• reference wavelength, the center of a wavelength band with the width of

reference bandwidth.

The signals comprises a series of data points over time, with the average

absorbance in the sample wavelength band minus the average absorbance of

the reference wavelength band.

Table 6 Peak Width — Response Time — Data Rate

Peak Width Response Time Data Rate

0.01 min 0.2 s 20 Hz

0.02 min 0.5 s 10 Hz

0.05 min 1.0 s 5 Hz

0.10 min 2.0 s 2.5 Hz

0.20 min 4.0 s 1.25 Hz

0.40 min 8.0 s 0.6 Hz

0.80 min 16.0 s 0.3 Hz

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Agilent Technologies 1100 Series Manual de servicio Pagina 36